Prohlížení dle autora "Gahura, Ondřej"
-
Characterization of Leucine aminopeptidase 1 in Trypanosoma brucei
Resl, Christian (Jihočeská univerzita, 2017) -
Characterization of mitochondrial proteins in Trypanosoma brucei
Svoboda, Lucia (Jihočeská univerzita, 2018)In this thesis proteins Tb927.2.3030 and Tb927.11.4870 are localized by in-situ tagging, im-munofluorescence microscopy and cell fractionation. Their essentiality for growth is deter-mined by downregulation with RNA interference. -
Iron-Sulfur Cluster Assembly in Trypanosoma brucei
Haindrich, Alexander Christoph (Jihočeská univerzita, 2013)In this thesis we investigated genes of the Cytosolic Iron sulfur cluster assembly (CIA) pathway in T. brucei procyclic and blood-stream form for their possible functional redundancy. For this, RNAi double knockdown plasmids ... -
Late Steps in the cytosolic Iron-Sulfur Cluster Assembly in Trypanosoma brucei
Haindrich, Alexander Christoph (Jihočeská univerzita, 2016)The aim of this thesis was to investigate genes involved in the late steps of the Cytosolic Iron sulfur cluster Assembly (CIA) pathway in procyclic T. brucei, to determine their cellular localization and find their possible ... -
Post-transcriptional regulation of TbIF1 in life cycle of Trypanosoma brucei
Gratzl, Sascha (Jihočeská univerzita, 2020)TbIF1, a protein Inhibitor of F1-ATPase in Trypanosoma brucei, is expressed exclusively in the insect stage of the parasite. In the bloodstream form, TbIF1 is switched off, because its activity interferes with the essential ... -
Preparation of antibodies to determine the association of mitoribosomal complexes with mitochondrial membrane
Zellner, Laura (Jihočeská univerzita, 2021)The assembly pathways of mitoribosomes, descendants of bacterial ancestors producing proteins encoded by vestigial mitochondrial genomes, remains largely unknown. To shed light on structural features of a precursor of the ... -
Subcellular localization analysis of proteins non univocally located to the mitochondria of Trypanosoma brucei
Essmann, Stina (Jihočeská univerzita, 2021)In this thesis in situ tagging by parasite transfection, followed by Western Blot and immunofluorescence microscopy was performed for the analysis of the endogenous localization of prioritized Trypanosoma brucei proteins. ...