| dc.contributor.advisor | Ettrich, Rüdiger Horst | |
| dc.contributor.author | Sinha, Dhiraj | |
| dc.date.accessioned | 2021-12-08T12:55:08Z | |
| dc.date.available | 2021-12-08T12:55:08Z | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2016-12-15 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/33859 | |
| dc.format | 154 p. | |
| dc.format | 154 p. | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | Type I RM system | eng |
| dc.subject | EcoR124I | eng |
| dc.subject | Pentameric complex | eng |
| dc.subject | Motor subunit | eng |
| dc.subject | Helicases | eng |
| dc.subject | Interactions | eng |
| dc.subject | Domain motions | eng |
| dc.subject | Molecular dynamics | eng |
| dc.subject | Principal component analysis | eng |
| dc.subject | Signal transfer | eng |
| dc.subject | Translocase activity | eng |
| dc.subject | Endonuclease activity | eng |
| dc.title | Interdoménové a intradoménové interakce u motorové podjednotky EcoR124I: Výpočetní studie | cze |
| dc.title.alternative | Inter and intra domain interaction in the motor subunit of EcoR124I: A computational study | eng |
| dc.type | disertační práce | cze |
| dc.identifier.stag | 45938 | |
| dc.description.abstract-translated | EcoR124I is a Type I restrictionmodification (RM) enzyme and as such forms multifunctional pentameric complexes with DNA cleavage and ATP-dependent DNA translocation activities located on the motor subunit HsdR. When non-methylated invading DNA is recognized by the complex, two HsdR endonuclease/motor subunits start to translocate dsDNA without strand separation activity up to thousands base pairs towards the stationary enzyme while consuming 1 molecule of ATP per base pair advanced. Whenever translocation is stalled the HsdR subunits cleave the dsDNA nonspecifically far from recognition site. The X-ray crystal structure of HsdR of EcoR124I bound to ATP gave a first insight of structural/functional correlation in the HsdR subunit. The four domains within the subunit were found to be in a square planer arrangement. Computational modeling including molecular dynamics in combination with crystallography, point mutations, in vivo and in vitro assays reveals how interactions between these four domains contribute to ATP-dependent DNA translocation, DNA cleavage or inter-domain communication between the translocase and endonuclease activities. | eng |
| dc.date.accepted | 2017-02-06 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biofyzika | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Ph.D. | |
| dc.thesis.degree-program | Biofyzika | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Spiwok, Vojtěch | |
| dc.contributor.referee | Stockner, Thomas | |
| dc.contributor.referee | Venkatachalam, K. V. | |
