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Improved demethylation in ecological epigenetic experiments: Testing a simple and harmless foliar demethylation application

dc.contributor.authorDvořáková, Hana
dc.date.accessioned2023-03-07T11:04:18Z
dc.date.available2023-03-07T11:04:18Z
dc.date.issued2019
dc.date.submitted2019-01-10
dc.identifier.urihttps://dspace.jcu.cz/handle/20.500.14390/40900
dc.description.abstractSpray application of 5azacytidine on established plant seedlings was tested for its demethylating efficiency. It represents a novel method for plant experimental demethylation with a potentially lower negative impact on plant development compared to the traditional application of the demethylating agent through germination of seeds in its solution. Further, the 5azacytidine spray application was used in practice to erase the epigenetic memory in offspring of Taraxacum brevicorniculatum plants from different competitive conditions. The impact of parental competition on the juvenile phenotype was estimated by measuring growth related traits, while the experimental demethylation allowed for evaluating the significance of DNA methylation marks in bioticaly induced transgenerational effects in T. brevirorniculatum.cze
dc.format11 s
dc.format11 s
dc.language.isoeng
dc.publisherJihočeská univerzitacze
dc.rightsBez omezení
dc.subject5-azacytidinecze
dc.subjectcompetitioncze
dc.subjectdemethylationcze
dc.subjectDNA methylationcze
dc.subjectecological epigeneticscze
dc.subjectecologycze
dc.subjectplant memorycze
dc.subjectplant performancecze
dc.subjecttoxicitycze
dc.subjecttransgenerational effectscze
dc.subject5-azacytidineeng
dc.subjectcompetitioneng
dc.subjectdemethylationeng
dc.subjectDNA methylationeng
dc.subjectecological epigeneticseng
dc.subjectecologyeng
dc.subjectplant memoryeng
dc.subjectplant performanceeng
dc.subjecttoxicityeng
dc.subjecttransgenerational effectseng
dc.titleImproved demethylation in ecological epigenetic experiments: Testing a simple and harmless foliar demethylation applicationcze
dc.title.alternativeImproved demethylation in ecological epigenetic experiments: Testing a simple and harmless foliar demethylation applicationeng
dc.typerigorózní prácecze
dc.identifier.stag56630
dc.description.abstract-translatedSpray application of 5azacytidine on established plant seedlings was tested for its demethylating efficiency. It represents a novel method for plant experimental demethylation with a potentially lower negative impact on plant development compared to the traditional application of the demethylating agent through germination of seeds in its solution. Further, the 5azacytidine spray application was used in practice to erase the epigenetic memory in offspring of Taraxacum brevicorniculatum plants from different competitive conditions. The impact of parental competition on the juvenile phenotype was estimated by measuring growth related traits, while the experimental demethylation allowed for evaluating the significance of DNA methylation marks in bioticaly induced transgenerational effects in T. brevirorniculatum.eng
dc.date.accepted2019-01-21
dc.description.departmentPřírodovědecká fakultacze
dc.thesis.degree-disciplineBotanikacze
dc.thesis.degree-grantorJihočeská univerzita. Přírodovědecká fakultacze
dc.thesis.degree-nameRNDr.
dc.thesis.degree-programBotanikacze
dc.description.gradeDokončená práce s úspěšnou obhajoboucze


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