| dc.contributor.advisor | Koblížek, Michal | |
| dc.contributor.author | Mujakić, Izabela | |
| dc.date.accessioned | 2026-01-06T11:55:49Z | |
| dc.date.available | 2026-01-06T11:55:49Z | |
| dc.date.issued | 2023 | |
| dc.date.submitted | 2023-09-18 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/48699 | |
| dc.format | 151 p | |
| dc.format | 151 p | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | Gemmatimonadota | eng |
| dc.subject | metagenome | eng |
| dc.subject | Gemmatimonadetes | eng |
| dc.subject | MAGs | eng |
| dc.subject | anoxygenic phototrophs | eng |
| dc.subject | aquatic bacteria | eng |
| dc.subject | CARD-FISH | eng |
| dc.subject | freshwater ecology | eng |
| dc.title | Ecology of Gemmatimonadota | cze |
| dc.title.alternative | Ecology of Gemmatimonadota | eng |
| dc.type | disertační práce | cze |
| dc.identifier.stag | 52532 | |
| dc.description.abstract-translated | Phylum Gemmatimonadota is a common group present in many natural environments. Yet, this interesting bacterial group is rarely studied. Since its discovery 20 years ago, only six cultured species have been described. Abundances of Gemmatimonadota in various environments are usually low (<1%), with the exception of soils, where they are one of the more abundant phyla. Probably for this reason, at the onset of this work, most of the knowledge about Gemmatimonadota came from studies of soil environments, while information about their ecology in freshwater lakes was missing. An interesting discovery relating to the unexplored diversity of Gemmatimonadota in freshwaters was the isolation of the first phototrophic member of this group, Gemmatimonas phototrophica, which was cultured from a shallow freshwater lake. To learn more about the ecology of Gemmatimonadota in freshwaters, the main focus of this thesis was the analysis of their distribution and diversity in several freshwater lakes, with emphasis on presence of photoheterotrophic Gemmatimonadota. The potential ecological roles of this group, metabolic capabilities and general genomic characteristics have also been addressed, thereby allowing a broader scope comparison with Gemmatimonadota from other environments such as soil, marine waters, or wastewaters. | eng |
| dc.date.accepted | 2023-10-27 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Hydrobiology | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Ph.D. | |
| dc.thesis.degree-program | Biology | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Wagner-Döbler, Irene | |
| dc.contributor.referee | Zeng, Yonghui | |
| dc.description.defence | <p>The hybrid defense was held in the presence of all but one designated member (authorized absence of Dr. Sirová) of the committee and both opponents (Dr. Zeng was present on-line via Zoom).</p>
<p>Introduction of the committee and opponents, passing an attendance list</p>
<p>Introduction of the candidate by her supervisor</p>
<p>Presentation of the dissertation thesis by Izabela Mujakić in 30 minutes</p>
<p>Supervisor’s assessment by doc. Michal Koblížek</p>
<p>Opponent’s evaluation by Prof. Dr. Irene Wagner-Döbler – a brief summary, questions: How do you know for sure that AAPs can grow anaerobically? Was the reservoir hypolimnion anoxic/anaerobic? How can you enrich AAPs from environmental samples and what is the most challenging step to cultivate them? Have you looked at the other CO<sub>2</sub> fixation genes? What are the main problems with MAGs? Discuss the advantages and disadvantages of this bioinformatics tool.</p>
<p>Opponent’s evaluation by Dr. Yonghui Zeng – a brief summary; questions: Is culturing AAPs the way to study them? What role can horizontal gene transfer play in the development of phototrophy in AAPs? How can you tell whether bacteria are losing or gaining phototrophic gene clusters? How specific was your FISH probe for detecting AAPs? Why do you distinguish the epilimnion and a euphotic zone?</p>
<p>Questions from the committee:</p>
<p>How fast can AAPs grow in freshwater?</p>
<p>Have you found AAPs in rivers?</p>
<p> </p>
<p>Overall assessment: Izabela Mujakić has proven her ability to plan and conduct scientific research successfully and satisfactorily responded to all questions in the discussion.</p>
<p>Committee poll: 8 valid ballot papers: passed 8×, failed 0×.</p> | cze |
