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dc.contributor.advisorLukeš, Julius
dc.contributor.authorTripathi, Pragya
dc.date.accessioned2026-01-06T11:55:51Z
dc.date.available2026-01-06T11:55:51Z
dc.date.issued2023
dc.date.submitted2023-06-19
dc.identifier.urihttps://dspace.jcu.cz/handle/20.500.14390/48704
dc.language.isoeng
dc.publisherJihočeská univerzitacze
dc.rightsBez omezení
dc.titleCharacterization of RNA binding proteins in Diplonema papillatum by using individual-nucleotide resolution Cross-Linking and ImmunoPrecipitation technique (iCLIP)cze
dc.typedisertační prácecze
dc.identifier.stag53758
dc.date.accepted2023-06-21
dc.description.departmentPřírodovědecká fakultacze
dc.thesis.degree-disciplineMolecular and Cell Biology and Geneticscze
dc.thesis.degree-grantorJihočeská univerzita. Přírodovědecká fakultacze
dc.thesis.degree-namePh.D.
dc.thesis.degree-programMolecular and Cell Biologycze
dc.description.gradeDokončená práce s úspěšnou obhajoboucze
dc.contributor.refereeKraeva, Natalia
dc.contributor.refereeSobotka, Roman
dc.description.defence<p>Defense was held in presence with the following committee:&nbsp;&nbsp;doc. Mgr. Tomáš Doležal, Ph.D. -head, RNDr. Pavel Doležal, Ph.D. (BIOCEV), prof. Ing. Miroslav Oborník. Reviewers (both present): doc. Ing. Roman Sobotka, Ph.D. (Algatech)&nbsp;and&nbsp;Ing. Natalia Kraeva, Ph.D. (Univ. of Ostrava).</p> <p>1. Mentor Julius Lukeš read his statement to introduce student.</p> <p>2. Pragya presented her work in approx. 30 min.</p> <p>3. Reviewer Natalia Kraeva read her review and discuss with student:&nbsp;</p> <p style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small;">Q: why only c-tagging was performed? A: partially answered, clearly, but the reasons were not quite convincing (but that also included&nbsp;mentor&#39;s decisions in project)</p> <p style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small;">Q: What is known about intraflagellar&nbsp;transport signal sequences. A: partially answered</p> <p style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small;">Comment from reviewer: she&nbsp;appreciated mentor&#39;s statement, which helped her to see Pragya&#39;s importance&nbsp;in the project</p> <p style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small;">4. Reviewer R. Sobotka read parts of his report and discussed with the student, asked&nbsp;about the contribution of Pragya to co-first author publication:</p> <p style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small;">Q1: 1st part clearly answered, 2nd too, 3rd partially and was clarified by other members of the group</p> <p style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small;">Q2: clarified</p> <p style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small;">Q 3: answered ok, 2nd part with help of mentor</p> <p style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small;">Q4: clarified by other member of the team, 2nd part - both reviewers missed alignment, 3rd part ok</p> <p style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small;">Q5: clarified</p> <p style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small;">5. Questions from committee: Obornik: Q: Can culture conditions influence flagellum formation? Answered by other team member. P. Dolezal: Q: Can you knock out genes?&nbsp;Answered&nbsp;by other team members. T. Dolezal returned to Q1 of R. Sobotka - it was clarified by the student.</p> <p style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small;">6. Evaluation of the defense by committee (without public): it is obvious that this was largely a team work, Pragya played an important role especially with selected methodologies and she contributed significantly. Presentation was well appreciated. The main, co-first author paper is still being in decision process - PhD title should be formally awarded only after a formal acceptance of the paper.</p> <p style="color: rgb(34, 34, 34); font-family: Arial, Helvetica, sans-serif; font-size: small;">7. Voting - 5 valid votes, ALL&nbsp;for PASSED.</p>cze


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