Identifikace a sekvenování genomu nového viru infikujícího vojtěšku

Bečková, Martina

dc.contributor.advisor	Petrzik, Karel
dc.contributor.author	Bečková, Martina
dc.date.accessioned	2021-12-06T13:09:07Z
dc.date.available	2021-12-06T13:09:07Z
dc.date.issued	2010
dc.date.submitted	2010-04-30
dc.identifier.uri	https://dspace.jcu.cz/handle/20.500.14390/23479
dc.format	41
dc.format	41
dc.language.iso	cze
dc.publisher	Jihočeská univerzita	cze
dc.rights	Bez omezení
dc.subject	carlavirus	cze
dc.subject	Alfalfa latent virus	cze
dc.subject	Pea streak virus	cze
dc.subject	izolát V4	cze
dc.subject	vojtěška	cze
dc.subject	Medicago sativa	cze
dc.subject	sekvenace	cze
dc.subject	identifikace viru	cze
dc.subject	carlavirus	eng
dc.subject	Alfalfa latent virus	eng
dc.subject	Pea streak virus	eng
dc.subject	isolate V4	eng
dc.subject	lucerne	eng
dc.subject	Medicago sativa	eng
dc.subject	sequencing	eng
dc.subject	virus identification	eng
dc.title	Identifikace a sekvenování genomu nového viru infikujícího vojtěšku	cze
dc.title.alternative	Identification and sequencing genom of a new virus infecting lucerne	eng
dc.type	diplomová práce	cze
dc.identifier.stag	13533
dc.description.abstract-translated	Samples of lucerne plants characteristic with local necrosic lesions, leave malformation and yellow spots on leaves were investigated with transmission electron microscopy. Virus particles observed there were filamentous ones of 600 to 700 nm long. Nucleic acid was isolated, transcribed and amplified using PCR. Genus-specific primers were designed based on reverse genetics from the highly conserved genes for carlaviruses, potexviruses and potyviruses. Successful amplification with carlavirus-specific primers, sequencing and comparison with sequences in GenBank database revealed presence of a carlavirus. This was later identified by nucleotide sequence comparison as a new isolate V4 of Alfalfa latent virus. Specific primers for isolate V4 were designed in a coat protein position. Half of the genom of this virus was obtained with PCR and PCR modified amplifications and compared with sequences of Alfalfa latent virus and Pea streak virus from GenBank.	eng
dc.date.accepted	2010-06-02
dc.description.department	Přírodovědecká fakulta	cze
dc.thesis.degree-discipline	Experimentální biologie - specializace Genetika a genové inženýrství	cze
dc.thesis.degree-grantor	Jihočeská univerzita. Přírodovědecká fakulta	cze
dc.thesis.degree-name	Mgr.
dc.thesis.degree-program	Biologie	cze
dc.description.grade	Dokončená práce s úspěšnou obhajobou	cze
dc.contributor.referee	Lenz, Ondřej
dc.contributor.referee	Vlasák, Josef