| dc.contributor.advisor | Asahina-Jindrová, Masako | |
| dc.contributor.author | Pospěch, Alexandr | |
| dc.date.accessioned | 2021-12-06T13:09:47Z | |
| dc.date.available | 2021-12-06T13:09:47Z | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-01-05 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/23573 | |
| dc.format | 58 s, 6 s příloh | |
| dc.format | 58 s, 6 s příloh | |
| dc.language.iso | cze | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | Caenorhabditis elegans | eng |
| dc.subject | Nuclear receptor | eng |
| dc.subject | Chromatin immunoprecipitation | eng |
| dc.title | Attempts on chromatin immunoprecipitation with <i>C. elegans</i> nuclear receptor NHR-25 | cze |
| dc.title.alternative | Attempts on chromatin immunoprecipitation with <i>C. elegans</i> nuclear receptor NHR-25 | eng |
| dc.type | diplomová práce | cze |
| dc.identifier.stag | 9750 | |
| dc.description.abstract-translated | The aim of the work presented in this thesis was to establish chromatin immunoprecipitation
method in our laboratory as a tool to study target genes of the nuclear receptor NHR-25 in C.
elegans. Once the method is established, it will be also useful for studies of other DNA
binding proteins. ChIP was performed in transiently transfected cells HEK293 and analyzed
using PCR and qPCR. Although ChIP is typically used to find authentic target genes in the
cell or in organisms, testing protein-DNA interactions by ChIP in transient transfection
system (by transfecting both the expression vector of the protein of interest and a vector
containing potential binding sequence/promoter of the protein) can be useful as it serves as a
relatively quick tool to confirm the direct binding. Since the detection is by PCR, this method
is sensitive yet less costly non radioactive method to analyze protein-DNA interaction. For the
first step towards ChIP in C. elegans; pulling down tagged protein directly from the worm
was also performed as a preparation for in vivo analysis of NHR-25 regulated genes. | eng |
| dc.date.accepted | 2010-01-27 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Experimentální biologie - specializace Buněčná a vývojová biologie | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Mgr. | |
| dc.thesis.degree-program | Biologie | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Bartůněk, Petr | |
| dc.contributor.referee | Panicucci Zíková, Alena | |
