| dc.contributor.advisor | Pavingerová, Daniela | |
| dc.contributor.author | Skotnicová, Petra | |
| dc.date.accessioned | 2021-12-06T13:44:13Z | |
| dc.date.available | 2021-12-06T13:44:13Z | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-04-29 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/24047 | |
| dc.description.abstract | Cílem práce byla molekulární analýza rostlin rododendronů, získaných po transformaci geny nptII a gusA pomocí bakterie Agrobacterium tumefaciens. Geny byly detekovány pomocí polymerázové řetězové reakce (PCR) a Southernovy hybridizace. Přítomnost genu gusA byla zjišťována také fluorimetrickou a histochemickou metodou stanovení GUS aktivity. | cze |
| dc.format | 43 s. (62 087 znaků-bez mezer) | |
| dc.format | 43 s. (62 087 znaků-bez mezer) | |
| dc.language.iso | cze | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | rododendron | cze |
| dc.subject | Agrobacterium | cze |
| dc.subject | ?-glukuronidáza | cze |
| dc.subject | transformace | cze |
| dc.subject | PCR | cze |
| dc.subject | Southernova hybridizace | cze |
| dc.subject | selekce na kanamycinu | cze |
| dc.subject | Rhododendron | eng |
| dc.subject | Agrobacterium | eng |
| dc.subject | ?-glucuronidase | eng |
| dc.subject | transformation | eng |
| dc.subject | PCR | eng |
| dc.subject | Southern analysis | eng |
| dc.subject | kanamycin selection | eng |
| dc.title | Molekulární analýza transgenních rostlin rododendronů, získaných po transformaci vektorem 35SGUSint. | cze |
| dc.title.alternative | Molecular analysis of transgenic rhododendron plants obtained by transformation with 35SGUSint. construct | eng |
| dc.type | diplomová práce | cze |
| dc.identifier.stag | 18094 | |
| dc.description.abstract-translated | The goal of this thesis was molecular analysis of rhododendron plants obtained after transformation by Agrobacterium tumefaciens and subsequent selection on kanamycin. Presence of incorporated genes gusA and nptII was determined by polymerase chain reaction (PCR) and Southern analysis. GUS activity was determined by fluorimetric and histochemical assay too. | eng |
| dc.date.accepted | 2011-05-26 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Experimentální biologie - specializace Genetika a genové inženýrství | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Mgr. | |
| dc.thesis.degree-program | Biologie | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Čurn, Vladislav | |
| dc.contributor.referee | Vejsadová, Hana | |
