dc.contributor.advisor | Sehnal, František | |
dc.contributor.author | Mičulková, Klára | |
dc.date.accessioned | 2021-12-06T13:44:33Z | |
dc.date.available | 2021-12-06T13:44:33Z | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-04-26 | |
dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/24075 | |
dc.format | 61 s. | |
dc.format | 61 s. | |
dc.language.iso | cze | |
dc.publisher | Jihočeská univerzita | cze |
dc.rights | Bez omezení | |
dc.title | Příprava rekombinantních proteinů pro použití v tkáňových kulturách | cze |
dc.title.alternative | Preparation of racombinant proteins for use in tissue cultures | eng |
dc.type | diplomová práce | cze |
dc.identifier.stag | 21413 | |
dc.description.abstract-translated | cDNA derived from exons 2 and 3 of the Ser2 (Sericin 2) gene of Bombyx mori and encoding 121 amino acids was expressed in Escherichia coli as a fusion protein with hexahistidine to allow purification by affinity chromatogramy on Ni-resine column. Several expression systems were used and the codon usage was optimized but the yield of recombinant protein remained low. Commercial sericin extract was therefore used in assay with human embryonic stem cells (hESC) ? the extract in some cases reduced defects (amplification of centrosomes) in hESC proliferating under in vitro conditions. | eng |
dc.date.accepted | 2012-05-29 | |
dc.description.department | Přírodovědecká fakulta | cze |
dc.thesis.degree-discipline | Klinická biologie | cze |
dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
dc.thesis.degree-name | Mgr. | |
dc.thesis.degree-program | Biologie | cze |
dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
dc.contributor.referee | Vlasák, Josef | |
dc.contributor.referee | Žurovec, Michal | |