| dc.contributor.advisor | Matoušek, Jaroslav | |
| dc.contributor.author | Selinger, Martin | |
| dc.date.accessioned | 2021-12-06T14:05:48Z | |
| dc.date.available | 2021-12-06T14:05:48Z | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-04-26 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/24496 | |
| dc.format | 59 | |
| dc.format | 59 | |
| dc.language.iso | cze | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | Potato spindle tuber viroid (PSTVd) | eng |
| dc.subject | PTGS | eng |
| dc.subject | vsRNA | eng |
| dc.subject | leaf factory | eng |
| dc.subject | Sl-MYB | eng |
| dc.title | Studium exprese cílových mRNA při pospiviroidní patogenezi v systému "leaf factory" | cze |
| dc.title.alternative | Expression of target mRNAs during Popsipviroid pathogenesis in the "leaf factory" system | eng |
| dc.type | diplomová práce | cze |
| dc.identifier.stag | 27827 | |
| dc.description.abstract-translated | The aim of this work was to identify potential mRNA targets of PTGS triggered by viroid-derived small RNAs (vsRNAs) in PSTVd-infected tomato plants (S. lycopersicum L.). We selected 47 possible gene targets using data provided by Prof. Dr. Steger (Heinrich Heine Universität, Düsseldorf, Germany) - the list of 1633 possible target mRNAs from tomato based on vsRNA:mRNA duplex prediction. The vsRNA sequences were obtained by Illumina sequencing of small RNA libraries from healthy and PSTVd-infected tomato plants. By qRT-PCR analysis we identified 6 genes with significantly altered levels of mRNA in PSTVd-infected tomato plants: CUL1 (protein ubiquitination), ERF4 (transcription factor of abiotic stress signalling pathway), H/ACA1 (rRNA pseudouridylation), NPH3 (transcription factor of fototropic signalling pathway), Sl-MYB (transcription factor regulating leaf development) and TCP3 (transcription factor regulating leaf development). The binary vector pLV07 with inserted expression cassette containing coding sequence of Sl-MYB was prepared for experiments in ?leaf factory? system in N. benthamiana plants. Expression analyses in ?leaf factory? system after 1,5 DPI using qRT-PCR and RNA blots revealed strong inhibition of expression of Sl-MYB in leaf sectors infiltrated with severe PSTVd AS1 strain, while mild PSTVd QFA strain showed minimal change in expression comparing to control sectors. Moreover, the overexpression of Sl-MYB in leaf sectors resulted in development of necroses after 2,5-3 DPI, in presence of silencing suppressor p19 after 2 DPI. The development of necroses was largely inhibited in PSTVd AS1-infiltrated leaf sectors in comparison with PSTVd QFA- and control-infiltrated sectors. | eng |
| dc.date.accepted | 2013-05-28 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Experimentální biologie - specializace Genetika a genové inženýrství | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Mgr. | |
| dc.thesis.degree-program | Biologie | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Petrzik, Karel | |
| dc.contributor.referee | Vlasák, Josef | |
