| dc.contributor.advisor | Zíková, Alena | |
| dc.contributor.author | Váchová, Hana | |
| dc.date.accessioned | 2021-12-06T14:19:29Z | |
| dc.date.available | 2021-12-06T14:19:29Z | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-04-24 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/24797 | |
| dc.format | 47 | |
| dc.format | 47 | |
| dc.language.iso | cze | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | T. brucei | eng |
| dc.subject | RNAi | eng |
| dc.subject | FoF1 ATPase/ATP synthase | eng |
| dc.title | Functional analysis of novel F<sub>1</sub>-ATPase subunit in <i>Trypanosoma brucei</i> | cze |
| dc.title.alternative | Functional analysis of novel F<sub>1</sub>-ATPase subunit in <i>Trypanosoma brucei</i> | eng |
| dc.type | diplomová práce | cze |
| dc.identifier.stag | 37502 | |
| dc.description.abstract-translated | Although F1-ATPase is extremely conserved among organisms, a putative subunit p18 was identified in Trypanosoma brucei F1-ATPase complex. To explore its function in the procylic, bloodstream and dyskinetoplastic trypanosomes, three different RNAi cell lines were created. Upon p18 silencing the F1-moiety structural integrity was impaired suggesting that p18 is indeed a bona fide subunit of this complex. Since F1-ATPase is crucial for the bloodstream form survival, its potential inhibitor from the 4-oxopiperidine-3,5-dicarboxylates class (JK-11) was examined. JK-11 inhibited growth of the bloodstream trypanosomes, decreased mitochondrial membrane potential and reduced ATPase and ATP synthase activity in mitochondrial lysates. Our results suggest that JK-11 may act on FoF1-ATP synthase/ATPase and its inhibition may contribute to the cytotoxicity of this drug. | eng |
| dc.date.accepted | 2015-05-27 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Experimentální biologie - specializace Buněčná a vývojová biologie | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Mgr. | |
| dc.thesis.degree-program | Biologie | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Horáková, Eva | |
| dc.contributor.referee | Verner, Zdeněk | |
