| dc.contributor.advisor | Nebesářová, Jana | |
| dc.contributor.author | Tashlieva, Ayya | |
| dc.date.accessioned | 2022-03-08T14:23:54Z | |
| dc.date.available | 2022-03-08T14:23:54Z | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2017-12-13 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/38579 | |
| dc.format | 65 p. (95 823 symbols) | |
| dc.format | 65 p. (95 823 symbols) | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | CLEM | eng |
| dc.subject | fluorescence microscopy | eng |
| dc.subject | electron microscopy | eng |
| dc.subject | TEM | eng |
| dc.subject | ROI | eng |
| dc.subject | B.Burgdorferi | eng |
| dc.subject | GFP | eng |
| dc.subject | embedding media | eng |
| dc.subject | acrylic resin | eng |
| dc.subject | HPF | eng |
| dc.subject | FS protocol | eng |
| dc.subject | sample sectioning | eng |
| dc.subject | microhardness | eng |
| dc.title | Visualization of surface antigens by correlative microscopy | cze |
| dc.title.alternative | Visualization of surface antigens by correlative microscopy | eng |
| dc.type | diplomová práce | cze |
| dc.identifier.stag | 41764 | |
| dc.description.abstract-translated | Currently Correlative Light- and Electron Microscopy (CLEM) is widely used for high-resolution analysis by electron microscopy (EM) after imaging by fluorescence markers by light microscopy (LM).
The differences in sample preparation protocols for LM and EM create certain difficulties to precise correlation of FLM and TEM imaging. Fluorescent marker GFP that used in present work allows the study of cellular processes and detect ROI, while TEM is used for B.burgdorferi ultrastructure study.
The high-pressure freezing (HPF) procedure, freeze substitution (FS) protocol and the correct choice of resin compound for embedding strongly affects the fluorescence preservation. Therefore, acrylic resins with different water content were investigated as a comprehensible variant for cryofixed biological samples for CLEM technique. | eng |
| dc.date.accepted | 2018-01-18 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biofyzika | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Mgr. | |
| dc.thesis.degree-program | Biofyzika | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Fessl, Tomáš | |
| dc.contributor.referee | Kaňa, Radek | |
