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dc.contributor.advisorŠlouf, Václav
dc.contributor.authorŠímová, Ivana
dc.date.accessioned2023-03-07T11:04:02Z
dc.date.available2023-03-07T11:04:02Z
dc.date.issued2019
dc.date.submitted2019-04-17
dc.identifier.urihttps://dspace.jcu.cz/handle/20.500.14390/40884
dc.format60 s.
dc.format60 s.
dc.language.isoeng
dc.publisherJihočeská univerzitacze
dc.rightsBez omezení
dc.subjectcarotenoideng
dc.subjectICTeng
dc.subjectpump-probeeng
dc.subjectspectroscopyeng
dc.titleDeactivation of the Intramolecular Charge Transfer state in RC-LH1 complexes of genetically modified purple bacteria <i>Rhodobacter sphaeroides</i>cze
dc.title.alternativeDeactivation of the Intramolecular Charge Transfer state in RC-LH1 complexes of genetically modified purple bacteria <i>Rhodobacter sphaeroides</i>eng
dc.typediplomová prácecze
dc.identifier.stag53098
dc.description.abstract-translatedThe main task of this thesis is to find the ICT activator of a carotenoid spheroidenone in the pigment-protein complex LH1 of Rhodobacter sphaeroides. The ICT state is characterized by a red-shifted positive transient absorption signal around 750 - 800 nm. We hypothesize that the presence of the ICT state is associated with the pigment-protein interaction of a carotenoid keto group and specific amino acid in the protein. To perform the experiment, four mutant complexes with amino acids substitutions, which should result in decreased intensity of the ICT signal due to a weaker or missing pigment-protein interaction, were obtained and measured using femtosecond time resolved pump-probe spectroscopy.eng
dc.date.accepted2019-05-23
dc.description.departmentPřírodovědecká fakultacze
dc.thesis.degree-disciplineBiofyzikacze
dc.thesis.degree-grantorJihočeská univerzita. Přírodovědecká fakultacze
dc.thesis.degree-nameMgr.
dc.thesis.degree-programBiofyzikacze
dc.description.gradeDokončená práce s úspěšnou obhajoboucze
dc.contributor.refereeAlster, Jan
dc.contributor.refereePolívka, Tomáš


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