| dc.contributor.advisor | Franta, Zdeněk | |
| dc.contributor.author | Huszárová, Eliška | |
| dc.date.accessioned | 2025-03-06T08:06:20Z | |
| dc.date.available | 2025-03-06T08:06:20Z | |
| dc.date.issued | 2022 | |
| dc.date.submitted | 2021-12-13 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/46252 | |
| dc.description.abstract | Tick-borne encephalitis virus (TBEV) is an important human pathogen and as of now, there are no specific therapeutics to combat this disease. In vitro and in cell models are essential in studying the disease and in the pursuit of novel therapeutics. This thesis deals with the design and production of two genetically encoded cleavage reporters of TBEV NS2B-NS3 protease activity. The reporters were produced in E. coli and tested with recombinant NS2B-NS3 protease, as well as produced in mammalian cell lines and analyzed for fluorescence upon infection with TBEV. | cze |
| dc.format | 66 p. | |
| dc.format | 66 p. | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | TBEV | eng |
| dc.subject | NS2B-NS3 protease | eng |
| dc.subject | cleavage reporter | eng |
| dc.subject | PAmCherry | eng |
| dc.title | Design and production of TBEV specific cleavage reporter for in vitro and in cell studies | cze |
| dc.title.alternative | Design and production of TBEV specific cleavage reporter for in vitro and in cell studies | eng |
| dc.type | diplomová práce | cze |
| dc.identifier.stag | 63434 | |
| dc.description.abstract-translated | Tick-borne encephalitis virus (TBEV) is an important human pathogen and as of now, there are no specific therapeutics to combat this disease. In vitro and in cell models are essential in studying the disease and in the pursuit of novel therapeutics. This thesis deals with the design and production of two genetically encoded cleavage reporters of TBEV NS2B-NS3 protease activity. The reporters were produced in E. coli and tested with recombinant NS2B-NS3 protease, as well as produced in mammalian cell lines and analyzed for fluorescence upon infection with TBEV. | eng |
| dc.date.accepted | 2022-01-27 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biological Chemistry | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Mgr. | |
| dc.thesis.degree-program | Biological Chemistry | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Selinger, Martin | |
| dc.contributor.referee | Šíma, Radek | |
| dc.description.defence | <p>The defense was performed in a hybrid format - both in-person and online via MS TEAMS.</p>
<p>All the members of the commission were present. Prof. Grubhoffer welcomes the student and the commission. Ms. Kotounová introduced the theoretical background of the thesis - tick-borne encephalitis virus, its proteins, and primarily the studied protein NS3. Furthermore, the idea of the production of a reporter for the NS3 activity determination was presented. The reporter was produced in E. coli bacteria and purified. Experiments related to the production and description of the reporter, as well as expression of the reporter in cell lines, were described. The usability of the reporter to monitor the NS2B/NS3 protease activity was shown.</p>
<p>The supervisor and the opponents presented their reviews, Ms. Kotounová answered the questions.</p>
<p>Questions from the commission followed on the timeline of the changes in fluorescence (and thus protease activity), expression of reporters, publication of the results. Commission members voted by MS Forms ballot papers.</p>
<p>voting of the commission - 8x Excellent = final grade is excellent</p> | cze |
