Vývoj, optimalizace a validace analytické metody na stanovení kyseliny chikorové a dalších derivátů kyseliny kávové pomocí LC/MS
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Pazderková, Nela
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Jihočeská univerzita
Abstract
This thesis deals with the development, optimization, and validation of an analytical method for the determination of chicoric acid and other caffeic acid derivates using LC/MS. First, the development and optimization of the analytical method was conducted. The following parameters were selected for the optimization: mobile phase composition and flow rate injection volume capillary temperature, voltage S-lens and F-lens voltage, ion injection time, product ion selection, and collision energy. Then, the analytical method was successfully validated and tested for quantitative analysis in a range of Echinacea food matrixes - tea, tablet, and tincture.
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Keywords
Kyselina chikorová, kyselina kávová, kyselina kaftarová, kyselina chlorogenová, cynarin, echinakosid, antioxidanty, hmotnostní spektrometrie, kapalinová chromatografie, optimalizace, validace., Chicoric acid, caffeic acid, caftaric acid, chlorogenic acid, cynarin, echinacosid, antioxidants, mass spectrometry, liquid chromatography, optimization, validation.