Counteracting constitutive activity of Orai1 with an H171Y mutation

Abstract

The functions and structures of the Orai1-ANSGA mutation are still not completely known. To further investigate possible changes in pore structure, Orai1 ANSGA and H171Y in combination with different amino acids were used in cysteine-crosslinking studies, to see if the ANSGA and H171Y mutations affect the orientation of pore-lining residues.