Mass spectrometry in structural proteomics: study of viral protein complexes

Abstract

This thesis investigated the avian reovirus sigma NS protein and its interaction with RNA using mass spectrometric methods. The sigma NS structure is still not known; thus, hydrogen-deuterium exchange and cross-linking coupled to mass spectrometry were employed to understand protein conformation. This research provides insights into protein-RNA interactions and the obtained data will be used in future work to predict protein structure.