Utilising CRISPR-Cas9 mediated genome editing to C-terminally tag endogenous gene loci with fluorescent protein fusions in preimplantation mouse embryos
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Authors
Pekľanská, Miriama
Journal Title
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Publisher
Jihočeská univerzita
Abstract
Master´s thesis demonstrates the C-terminally fluorescent tagging of endogenous genes in the preimplantation mouse embryos by utilizing CRISPR-Cas9 mediated genome editing method.
Description
Keywords
mouse preimplantation developmental stages, the first cell-fate decision, the second cell-fate decision, mCherry, WWC2, p38-Mapks, CRISPR-Cas9, fluorescent C-terminal tagging., mouse preimplantation developmental stages, the first cell-fate decision, the second cell-fate decision, mCherry, WWC2, p38-Mapks, CRISPR-Cas9, fluorescent C-terminal tagging.