CRISPR/Cas9 Genome editing in Pyrrhocoris apterus
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Authors
Bertolutti, Maly
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Publisher
Jihočeská univerzita
Abstract
The CRISPR/Cas9 technology is a cutting edge method to genetically modify specific targets in the genome, which allows to create mutants in non-model organisms. The aim of this project was to genetically modify the Pyrrhocoris apterus genomic sequence of the cryptochrome2 gene using the CRISPR/Cas9 technology. Specifically, this project aimed to create a mutant with removed or modified C-terminal part of the CRY2 protein, which should later allow for studying the role of this sequence in CRY2 function.
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Keywords
genome editing, Pyrrhocoris apterus, CRISPR technology, CRISPR/Cas9, CRY2, cryptochrome, chryptochrome gene, ciradian clock, photoperiodic clock, entomology, genome editing, Pyrrhocoris apterus, CRISPR technology, CRISPR/Cas9, CRY2, cryptochrome, chryptochrome gene, ciradian clock, photoperiodic clock, entomology