| dc.contributor.advisor | Trantírková, Silvie | |
| dc.contributor.author | Matulová, Jana | |
| dc.date.accessioned | 2021-12-06T12:31:05Z | |
| dc.date.available | 2021-12-06T12:31:05Z | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-05-09 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/22859 | |
| dc.format | 40 s. | |
| dc.format | 40 s. | |
| dc.language.iso | cze | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.title | Studium vlivu posttranslačních modifikací na strukturu proteinu za využití <i>Leishmania tarentolae</i> jako expresního systému: vývoj metodiky isotopického značení proteinů | cze |
| dc.title.alternative | Influence of posttranslation modifications on protein structure: development of methodology for isotopic labeling of proteins in <i>Leishmania tarentolae</i> expression system | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 4006 | |
| dc.description.abstract-translated | We have established a cost efficient approach for isotopic labeling of proteins using Leishmania tarentolae expression system. The method is based on cultivation L. tarentolae in a rich complex (BHI) medium supplemented with labeled amino acid(s). This approach leads to low level isotope enrichment, which implies the decreased sensitivity of NMR experiment. In the same time this approach leads to a two to three fold increase of the yield of the recombinant protein. Importantly, it leads to 5 - 10 fold reduction of the overall costs as compared to the recently established protocol (Niculae et al., 2006).
In parallel, we have attempted to estimate the suitability of L. taretolae for in-cell NMR spectroscopy. | eng |
| dc.date.accepted | 2007-06-07 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biomedicínská laboratorní technika | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biologie | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Vácha, František | |
