| dc.contributor.advisor | Marec, František | |
| dc.contributor.author | Šíchová, Jindra | |
| dc.date.accessioned | 2021-12-06T13:08:04Z | |
| dc.date.available | 2021-12-06T13:08:04Z | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-01-06 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/23391 | |
| dc.format | 35 s., 10 s. obr. příloh | |
| dc.format | 35 s., 10 s. obr. příloh | |
| dc.language.iso | cze | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.title | Analýza pohlavního chromosomu Z obaleče jablečného(<i>Cydia pomonella</i>) pomocí laserové mikrodisekce | cze |
| dc.title.alternative | Analysis of the codling moth (<i>Cydia pomonella</i>) Z chromosome by means of laser microdissection. | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 6309 | |
| dc.description.abstract-translated | This study uses a new methodical approach for the molecular analysis of the lepidopteran Z chromosome. The Z-chromosome DNA was collected from male mitotic spermatogonial cells of the codling moth (Cydia pomonella) by laser microdissection and then amplified by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). Products of DOP-PCR were used to prepare painting probes and create a Z-chromosome DNA sequence library. Specificity of painting probes was tested on spread chromosomal preparations from testes and ovaries of the codling moth larvae using fluorescence in situ hybridization (FISH). However, all painting probes made exhibited a low specificity for the Z chromosome: they hybridized evenly to all chromosomes in male preparations, whereas in female preparations highlighted almost the entire W chromosome. For construction of the Z-chromosome DNA library, DOP-PCR-generated DNA fragments were cloned, sequenced and further analyzed. A total of 30 different sequences were obtained. Only four of the sequences exhibited a significant homology to sequences in GenBank, all other represent unknown sequences. Five sequences were characterized by Southern hybridization. The results correlated with hybridization signals from FISH; we recovered highly repetitive sequences, either ubiquitous(i.e. occurring in all chromosomes) and/or accumulated on the W chromosome. Only a few copies were located on Z chromosome. | eng |
| dc.date.accepted | 2009-01-29 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biologie | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biologie | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Šťáhlavský, František | |
