| dc.contributor.advisor | Bříza, Jindřich | |
| dc.contributor.author | Kolářová, Klára | |
| dc.date.accessioned | 2021-12-06T13:08:36Z | |
| dc.date.available | 2021-12-06T13:08:36Z | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-04-30 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/23437 | |
| dc.description.abstract | Cílem této práce bylo optimalizovat podmínky pro transformaci embryogenní kultury smrku ztepilého (Picea abies) pomocí mikroprojektilů.Použitým slekčním systémem byl gen nptII v kombinaci s antibiotikem kanamycinem. Tranzientní exprese genu gus byla testována metodou histochemického stanovení tohoto genu. | cze |
| dc.format | 50 s | |
| dc.format | 50 s | |
| dc.language.iso | cze | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | transformace | cze |
| dc.subject | Picea abies | cze |
| dc.subject | mikroprojektily | cze |
| dc.subject | embryogenní kultura | cze |
| dc.subject | smrk ztepilý | cze |
| dc.subject | genetic transformation | eng |
| dc.subject | Picea abies | eng |
| dc.subject | particle bombardment | eng |
| dc.subject | embryogenic cultures | eng |
| dc.subject | Norway spruce | eng |
| dc.title | Transformace embryogenní kultury smrku ztepilého (<i>Picea abies</i>) | cze |
| dc.title.alternative | Genetic transformation of embryogenic cultures of Norway spruce (<i>Picea abies</i>) | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 9790 | |
| dc.description.abstract-translated | The aim of this study was to develop a transformation procedure for embryonic cultures of Norway spruce (Picea abies) using particle bombardment. Selection of the transgenic tissue was based on the antibiotic resistance conferring by the nptII gene, in combination with the antibiotic kanamycin. Expression of the gus gene was confirmed by histochemical analysis. | eng |
| dc.date.accepted | 2009-06-02 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biologie | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biologie | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Rakouský, Slavomír | |
