| dc.contributor.advisor | Kopáček, Petr | |
| dc.contributor.author | Rathner, Petr | |
| dc.date.accessioned | 2021-12-06T13:40:22Z | |
| dc.date.available | 2021-12-06T13:40:22Z | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-05-20 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/23784 | |
| dc.format | 24 s. (5149 znaků) | |
| dc.format | 24 s. (5149 znaků) | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | MCR | eng |
| dc.subject | IrAM9 | eng |
| dc.subject | ?2-macroglobulin | eng |
| dc.subject | Ixodes | eng |
| dc.subject | ricinus | eng |
| dc.title | IrAM9 - a member of a thioester-containing protein family from the hard tick <i>Ixodes ricinus</i> | cze |
| dc.title.alternative | IrAM9 - a member of a thioester-containing protein family from the hard tick <i>Ixodes ricinus</i> | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 19687 | |
| dc.description.abstract-translated | The thioester-containing proteins (proteins of ?2-macroglobulin family) are constituents of innate immunity, which comprise (i) universal protease inhibitors of ?2M type, (ii) C3, C4, C5 components of the complement system, (iii) insect thioester-contaning proteins (iTEPs) and (iv) macroglobulin complement related proteins (MCR). In this work, the partial structure of the thioester-containing protein from the hard tick Ixodes ricinus (IrAM9) which belong to the MCR was determined by sequencing and cloning of PCR products. The IrAM9 message is expressed in the ovaries and salivary glands of partially engorged females. Received recombinant protein was used to get polyclonal serum which was obtained by repeated rabbit immunization. The obtained antibodies were used in an attempt to detect the native protein in tick tissues by immunoblotting method. | eng |
| dc.date.accepted | 2011-06-20 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biological Chemistry | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biochemistry | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Sojka, Daniel | |
