| dc.contributor.advisor | Štěrba, Ján | |
| dc.contributor.author | Kočová, Pavlína | |
| dc.date.accessioned | 2021-12-06T14:03:46Z | |
| dc.date.available | 2021-12-06T14:03:46Z | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-04-24 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/24341 | |
| dc.format | 37 s. | |
| dc.format | 37 s. | |
| dc.language.iso | cze | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | bioortogonální chemie | cze |
| dc.subject | BONCAT | cze |
| dc.subject | klíšťová encefalitida | cze |
| dc.subject | bioorthogonal chemistry | eng |
| dc.subject | BONCAT | eng |
| dc.subject | tick-borne encephalitis | eng |
| dc.title | Identifikace diferenciálně exprimovaných proteinů v savčích buňkách v průběhu infekce virem s využitím bioortogonální chemie (L-azidohomoalaninu) | cze |
| dc.title.alternative | Identification of newly synthesised proteins in mammalian cells during tick-borne encephalitis infection using bioorthogonal chemistry (L-azidohomoalanine) | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 35442 | |
| dc.description.abstract-translated | This study was focused on changes in protein expression in glioblastoma cell line after infection with tick-borne encephalitis virus. Bioorthogonal chemistry (BONCAT method) was used to distinguish between previously synthesized proteins and newly synthesized proteins to observe changes in protein synthesis. Labelled proteins were purified and visualized using SDS-PAGE and western blotting. Purified proteins were also indentified using mass spectrometry. The number of the different labelled proteins decreases as the infection progress. | eng |
| dc.date.accepted | 2014-05-29 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biomedicínská laboratorní technika | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biologie | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Lieskovská, Jaroslava | |
