Molecular and biochemical characterization of serine protease SmSP1 in <i>Schistosoma mansoni</i>
Abstrakt
SmSP1 is a chimerical serine protease consisted of three domains (cub, LDLa
and trypsin-like) and found in Schistosoma mansoni. Its characterization was performed
by molecular techniques such as PCR screen, qRT-PCR and RNA interference (RNAi) to
gain information about expression profile, level expression and susceptibility to RNAi.
Further, protein expression was carried out to gain an antigen for immunization and
recombinant for biochemical studies. Results of PCR screen and qRT-PCR suggested
possible function of SmSP1 in egg and adult stages but SmSP1 gene was not found
susceptible to RNAi in NTS. Recombinant from E. coli was successfully used for
immunization. Active recombinant was likely expressed in Pichia pastoris but expression
conditions are unstable and expression optimization is necessary.