Creation of Drosophila melanogaster mutants for multiple sirtuin genes (Sirt2, Sirt6 and Sirt7)

Abstract

The aim of this thesis was to create a fruit fly that is a mutant for multiple sirtuin genes, Sirt2,Sirt6 and Sirt7, by meiotic recombination and Crispr/Cas 9 technology. This fly would be used in order to achieve the creation of a fly mutant for all sirtuin genes, whose phenotype would be characterized by testing it under stress conditions.