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dc.contributor.advisorTušerová, Jindra
dc.contributor.authorPospíšilová, Kristýna
dc.date.accessioned2022-03-08T14:21:33Z
dc.date.available2022-03-08T14:21:33Z
dc.date.issued2018
dc.date.submitted2018-04-17
dc.identifier.urihttps://dspace.jcu.cz/handle/20.500.14390/38481
dc.format30
dc.format30
dc.language.isoeng
dc.publisherJihočeská univerzitacze
dc.rightsBez omezení
dc.subjectLepidopteraeng
dc.subjectLeptidea wood white butterflieseng
dc.subjectMultiple sex chromosomeseng
dc.subjectFluorescence in situ hybridizationeng
dc.subjectChromosomal rearrangementseng
dc.subjectSpeciationeng
dc.titleAnalysis of structure and origin of multiple sex chromosomes in <i>Leptidea</i> wood white butterfliescze
dc.title.alternativeAnalysis of structure and origin of multiple sex chromosomes in <i>Leptidea</i> wood white butterflieseng
dc.typebakalářská prácecze
dc.identifier.stag48971
dc.description.abstract-translatedPrevious studies have shown a dynamic karyotype evolution and the presence of complex sex chromosome systems with 3-4 W chromosomes and 3-6 Z chromosomes in Leptidea wood white butterflies. To dissect the evolutionary history of multiple Z chromosomes of Leptidea species, we used identified and selected bacterial artificial chromosome (BAC) clones containing orthologous genes of Bombyx mori chromosome Z and 17, isolated them from the available BAC library and used them as probes for physical mapping by BAC-FISH, first in L. juvernica, then in the closely related L. sinapis. In both Leptidea species, the majority of BAC clones corresponding to the linkage group Z of the B. mori reference genome hybridized to one chromosome of the complicated sex chromosome multivalent. Thus, we named it as Z1 chromosome. Location of all Z-derived BAC clones was identical in both species suggesting a conserved synteny and gene order between L. juvernica and L. sinapis Z1 chromosome. Moreover, our findings indicate that the Z1 chromosome is probably the ancestral Z chromosome in the genus Leptidea. Results of BAC-FISH mapping with clones corresponding to the linkage group 17 of the B. mori reference genome revealed the fusion/translocation event between an ancestral Z chromosome and the chromosome corresponding to B. mori chromosome 17 and supported a previous hypothesis about the role of chromosomal rearrangements in the formation of multiple sex chromosomes in Leptidea butterflies.eng
dc.date.accepted2018-05-23
dc.description.departmentPřírodovědecká fakultacze
dc.thesis.degree-disciplineBiologiecze
dc.thesis.degree-grantorJihočeská univerzita. Přírodovědecká fakultacze
dc.thesis.degree-nameBc.
dc.thesis.degree-programBiologiecze
dc.description.gradeDokončená práce s úspěšnou obhajoboucze
dc.contributor.refereeMajtánová, Zuzana


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