| dc.contributor.advisor | Štěrba, Ján | |
| dc.contributor.author | Stemmer, Vitus | |
| dc.date.accessioned | 2024-03-12T08:12:17Z | |
| dc.date.available | 2024-03-12T08:12:17Z | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-08-13 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/42729 | |
| dc.description.abstract | A recombinant enzyme PNGase H+ was produced in E. coli, and several steps were tried to optimize the expression, extraction and purification. Furthermore, PCR primers were designed to detect genes of predicted sialyltransferases, fucosyltransferases and DNA methyltransferases in Ixodes ricinus genomic DNA and plasmids were designed for future production of DNA methyltransferases in an insect expression system. | cze |
| dc.format | 33 p. (53 122 characters) | |
| dc.format | 33 p. (53 122 characters) | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.title | Production of recombinant proteins using prokaryotic and eukaryotic expression systems | cze |
| dc.title.alternative | Production of recombinant proteins using prokaryotic and eukaryotic expression systems | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 56188 | |
| dc.description.abstract-translated | A recombinant enzyme PNGase H+ was produced in E. coli, and several steps were tried to optimize the expression, extraction and purification. Furthermore, PCR primers were designed to detect genes of predicted sialyltransferases, fucosyltransferases and DNA methyltransferases in Ixodes ricinus genomic DNA and plasmids were designed for future production of DNA methyltransferases in an insect expression system. | eng |
| dc.date.accepted | 2020-09-17 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biological Chemistry | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biochemistry | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Grinkevich, Pavel | |
