| dc.contributor.advisor | Durante, Ignacio Miguel | |
| dc.contributor.author | Essmann, Stina | |
| dc.date.accessioned | 2024-03-12T11:36:03Z | |
| dc.date.available | 2024-03-12T11:36:03Z | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-08-24 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/44850 | |
| dc.description.abstract | In this thesis in situ tagging by parasite transfection, followed by Western Blot and immunofluorescence microscopy was performed for the analysis of the endogenous localization of prioritized Trypanosoma brucei proteins. The prioritized non univocally located proteins had TrypTag.org annotations that corresponded or were compatible with tripartite attachment complex (TAC). For the determination of essentiality, RNAi constructs were generated by Gibson assembly molecular cloning. | cze |
| dc.format | 43 | |
| dc.format | 43 | |
| dc.language.iso | xx | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | T. brucei | cze |
| dc.subject | Western Blot | cze |
| dc.subject | putative proteins | cze |
| dc.subject | IFA | cze |
| dc.subject | localization | cze |
| dc.subject | mitochondrial | cze |
| dc.subject | cytoplasmic | cze |
| dc.subject | T. brucei | eng |
| dc.subject | Western Blot | eng |
| dc.subject | putative proteins | eng |
| dc.subject | IFA | eng |
| dc.subject | localization | eng |
| dc.subject | mitochondrial | eng |
| dc.subject | cytoplasmic | eng |
| dc.title | Subcellular localization analysis of proteins non univocally located to the mitochondria of Trypanosoma brucei | cze |
| dc.title.alternative | Subcellular localization analysis of proteins non univocally located to the mitochondria of Trypanosoma brucei | eng |
| dc.title.alternative | - | cze |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 66329 | |
| dc.description.abstract-translated | In this thesis in situ tagging by parasite transfection, followed by Western Blot and immunofluorescence microscopy was performed for the analysis of the endogenous localization of prioritized Trypanosoma brucei proteins. The prioritized non univocally located proteins had TrypTag.org annotations that corresponded or were compatible with tripartite attachment complex (TAC). For the determination of essentiality, RNAi constructs were generated by Gibson assembly molecular cloning. | eng |
| dc.date.accepted | 2021-09-16 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biological Chemistry | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biochemistry | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Franta, Zdeněk | |
| dc.contributor.referee | Gahura, Ondřej | |
