| dc.contributor.advisor | Rego, Ryan Oliver Marino | |
| dc.contributor.author | Krátká, Julie | |
| dc.date.accessioned | 2025-03-06T08:05:36Z | |
| dc.date.available | 2025-03-06T08:05:36Z | |
| dc.date.issued | 2022 | |
| dc.date.submitted | 2021-12-15 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/46145 | |
| dc.description.abstract | From Ixodes ricinus was extracted cDNA containing gene with a defensin. The defensin was cloned and expressed in modified E. coli bacteria. Results were analyzed via Western blot technique. Peptides used for testing of antibacterial activity were obtained from I. ricinus and added into borrelia cultures to observe their bactericidal effect against borrelia. | cze |
| dc.format | 35 | |
| dc.format | 35 | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | Ixodes ricinus | cze |
| dc.subject | defensins | cze |
| dc.subject | antimicrobial peptides | cze |
| dc.subject | Borrelia | cze |
| dc.subject | antibacterial activity | cze |
| dc.subject | Ixodes ricinus | eng |
| dc.subject | defensins | eng |
| dc.subject | antimicrobial peptides | eng |
| dc.subject | Borrelia | eng |
| dc.subject | antibacterial activity | eng |
| dc.title | Expression and characterization of defensins from the hard tick Ixodes ricinus | cze |
| dc.title.alternative | Expression and characterization of defensins from the hard tick Ixodes ricinus | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 59992 | |
| dc.description.abstract-translated | From Ixodes ricinus was extracted cDNA containing gene with a defensin. The defensin was cloned and expressed in modified E. coli bacteria. Results were analyzed via Western blot technique. Peptides used for testing of antibacterial activity were obtained from I. ricinus and added into borrelia cultures to observe their bactericidal effect against borrelia. | eng |
| dc.date.accepted | 2022-01-24 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biological Chemistry | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biochemistry | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Tonk, Miray | |
| dc.description.defence | <ul>
<li>Defence was hybrid, the student was present in person, the members of the commission were connected via MS Teams.</li>
<li>Prof. Grubhoffer welcomed the student and the commission.</li>
<li>The student presented her work „Expression and characterization of defensins from the hard tick <em>Ixodes ricinus</em>“, introduction to the topic, methods, results and future perspectives.</li>
<li>Supervisor Dr. Rego presented his review.</li>
<li>Dr. Štěrba presented Dr. Tonk´s opponent review.</li>
<li>Student answered opponent´s questions on cathegories of AMPs, cloning of DefMT3, protein production expression systems, the biggest challenge for the student.</li>
<li>Dr. Štěrba presented Dr. Tonk´s comments on the thesis.</li>
<li>Student answered questions of the members of the commission:</li>
</ul>
<p>Dr. Prudnikova – the size of the peptides, optimization of the protein expression</p>
<p>Dr. Štěrba – cloning problems</p>
<p> </p>
<p>3x excellent, 1x very good = excellent</p> | cze |
