| dc.contributor.advisor | Frischauf, Irene | |
| dc.contributor.author | Marko, Ricarda | |
| dc.date.accessioned | 2025-03-06T08:05:49Z | |
| dc.date.available | 2025-03-06T08:05:49Z | |
| dc.date.issued | 2022 | |
| dc.date.submitted | 2021-10-22 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/46193 | |
| dc.description.abstract | The functions and structures of the Orai1-ANSGA mutation are still not completely known. To
further investigate possible changes in pore structure, Orai1 ANSGA and H171Y in
combination with different amino acids were used in cysteine-crosslinking studies, to see if the
ANSGA and H171Y mutations affect the orientation of pore-lining residues. | cze |
| dc.format | 29 | |
| dc.format | 29 | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | Ion channels | cze |
| dc.subject | Ca2+ channels | cze |
| dc.subject | Orai1 | cze |
| dc.subject | ANSGA | cze |
| dc.subject | cysteine-crosslinking | cze |
| dc.subject | H171Y | cze |
| dc.title | Counteracting constitutive activity of Orai1 with an
H171Y mutation | cze |
| dc.title.alternative | Counteracting constitutive activity of Orai1 with an
H171Y mutation | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 66111 | |
| dc.date.accepted | 2022-01-24 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biological Chemistry | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biochemistry | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Franta, Zdeněk | |
| dc.description.defence | <ul>
<li>Defence was hybrid, the student was present in person, the members of the commission were present in person or online via MS Teams.</li>
<li>Prof. Grubhoffer welcomed the student and the commission.</li>
<li>The student presented her work „Counteracting constitutive activity of Orai1 with an H171Y mutation“, introduction to the topic, methods, results and summary.</li>
<li>Supervisor Dr. Frischauf presented her review.</li>
<li>Oponnent Dr. Franta presented his review.</li>
<li>Student answered opponent´s questions on polymerase control in PCR reaction, DNA template for PCR reaction, vector resistance, E.coli genotype, mutations, student contribution to the production of mutations</li>
<li>Student discussed oponnent´s questions with the oponnent on cloning procedure, identification of proteins by Western Blot analysis, test of the specific antibody, calculation of the protein molecular weight from the sequence, activity measurement</li>
<li>Oponnent presented his comments on the thesis.</li>
<li>Student discussed questions with the members of the commission:</li>
</ul>
<p>Dr. Zíková – reducing agent to disrupt disulfide bond</p>
<p>Dr. Štěrba – polymerase in PCR reaction</p>
<p> </p>
<p> </p>
<p>1x very good, 2x good, 2x failed = good</p> | cze |
