| dc.contributor.advisor | Jalovecká, Marie | |
| dc.contributor.author | Křečková, Tereza | |
| dc.date.accessioned | 2025-03-06T08:05:56Z | |
| dc.date.available | 2025-03-06T08:05:56Z | |
| dc.date.issued | 2022 | |
| dc.date.submitted | 2022-04-13 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/46213 | |
| dc.format | 66 | |
| dc.format | 66 | |
| dc.language.iso | cze | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | Babesia divergens | cze |
| dc.subject | kalcium-dependentní proteinkinázy | cze |
| dc.subject | CDPKs | cze |
| dc.subject | Babesia divergens | eng |
| dc.subject | Calcium-dependent proteinkinases | eng |
| dc.subject | CDPKs | eng |
| dc.title | Pilotní analýza kalcium-dependentních proteinkináz
prvoka <i>Babesia divergens</i> | cze |
| dc.title.alternative | Pilot Analysis of <i>Babesia divergens</i> Calcium-Dependent Protein Kinases | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 66566 | |
| dc.description.abstract-translated | Babesia divergens is a parasite of the Apicomplexa that causes bovine and human
babesiosis primarily in Europe. The ability of B. divergens to parasitize inside the host red blood
cells is governed by the Ca2+ signaling pathway employing various molecules including
an array of Ca2+
-dependent protein kinases (CDPKs). These enzymes are orchestrating essential
developmental processes of Apicomplexa parasites, and since they are absent in their animal
host, they possess a great potential for therapeutic interventions. Despite CDPKs' vital
and promising role, Babesia CDPKs remain uncharacterized, and their potential regulatory
roles in host-cell invasion and intracellular parasitism are thus not known. This thesis is aimed
at the pilot analysis of four putative B. divergens-specific CDPKs: Bdiv_014540c,
Bdiv_024410, Bdiv_030560 and Bdiv_035940c. We amplified and sequenced full genomic
and coding sequences of putative BdCDPKs and inserted their coding sequences into a standard
vector. Using BdCDPKs protein sequences we analyzed the architecture of the individual
enzyme to confirm the presence of key CDPKs domains (Ser-Thr kinase domain and EF-hands)
and constructed a phylogenetic tree to confirm the clustering of putative BdCDPKs with
analogous enzymes of other Apicomplexa and their relatives. Overall, the pilot analysis
of BdCDPKs presented in this thesis provides important background for further functional
and biochemical analyses of these yet uncharacterized but highly important B. divergens
enzymes. | eng |
| dc.date.accepted | 2022-05-26 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biologie | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biologie | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Fiala, Ivan | |
| dc.description.defence | <p>Studentka seznámila komisi s výsledky své bakalářské práce. Školitelka a oponent přečetli své posudky. Studentka odpověděla na vznesené dotazy. Následovala diskuse: metodická témata, fylogeneze babesií, porovnání znalostí o jednotlivých skupinách.</p>
<p>145 bodů</p> | cze |
