| dc.contributor.advisor | Bárta, Jiří | |
| dc.contributor.author | Turková, Sára | |
| dc.date.accessioned | 2025-03-06T08:58:36Z | |
| dc.date.available | 2025-03-06T08:58:36Z | |
| dc.date.issued | 2022 | |
| dc.date.submitted | 2022-05-11 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/46849 | |
| dc.description.abstract | The gene abundances and community structures of nitrogen-fixing and denitrifying bacteria in permafrost-affected soils with different moisture level were analysed. Additionally, DNA- and RNA-based analysis was used to evaluate the variations in gene copy numbers and community composition between the total and active bacteria. The gene abundances of nitrogen-fixing and denitrifying bacteria were quantified by qPCR of the nifH and nosZ genes, and the gene copy numbers were compared between the soils with different moisture. Furthermore, the bacterial community structure variation was evaluated by analysis of the taxonomic classification obtained from sequencing data. | cze |
| dc.format | 30 p. | |
| dc.format | 30 p. | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | Arctic | cze |
| dc.subject | permafrost thaw | cze |
| dc.subject | soil moisture | cze |
| dc.subject | nitrogen cycle | cze |
| dc.subject | nifH | cze |
| dc.subject | nosZ | cze |
| dc.subject | gene abundance | cze |
| dc.subject | qPCR | cze |
| dc.subject | bacterial community structure | cze |
| dc.subject | Arctic | eng |
| dc.subject | permafrost thaw | eng |
| dc.subject | soil moisture | eng |
| dc.subject | nitrogen cycle | eng |
| dc.subject | nifH | eng |
| dc.subject | nosZ | eng |
| dc.subject | gene abundance | eng |
| dc.subject | qPCR | eng |
| dc.subject | bacterial community structure | eng |
| dc.title | Effect of different water regime on microorganisms of nitrogen cycle in permafrost | cze |
| dc.title.alternative | Effect of different water regime on microorganisms of nitrogen cycle in permafrost | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 63484 | |
| dc.description.abstract-translated | The gene abundances and community structures of nitrogen-fixing and denitrifying bacteria in permafrost-affected soils with different moisture level were analysed. Additionally, DNA- and RNA-based analysis was used to evaluate the variations in gene copy numbers and community composition between the total and active bacteria. The gene abundances of nitrogen-fixing and denitrifying bacteria were quantified by qPCR of the nifH and nosZ genes, and the gene copy numbers were compared between the soils with different moisture. Furthermore, the bacterial community structure variation was evaluated by analysis of the taxonomic classification obtained from sequencing data. | eng |
| dc.date.accepted | 2022-06-15 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biological Chemistry | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biological Chemistry | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.description.defence | <p>The Head of the Commission welcomed the student, commission members, and the audience. The student explained the basics of permafrost biology, the nitrogen cycle and the aims of the thesis. Next, the results of the work were presented and discussed.</p>
<p>The supervisor and the opponent presented their reviews and the student answered the questions. Next, she did not answer completely any of the questions from the commission on the various aspects of the analyses, methodology, experimental setup etc.</p>
<p>3x excellent, 2x very good, final - excellent</p> | cze |
