| dc.contributor.advisor | Angel, Roey | |
| dc.contributor.author | Vranjes, Sebastian | |
| dc.date.accessioned | 2025-03-06T08:58:38Z | |
| dc.date.available | 2025-03-06T08:58:38Z | |
| dc.date.issued | 2022 | |
| dc.date.submitted | 2022-08-24 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/46859 | |
| dc.description.abstract | The aim of this study is to establish a correction factor for ddPCR, accounting for instances of the target sequence's occurrences being miscounted due to endpoint detection limitations. | cze |
| dc.format | 21 | |
| dc.format | 21 | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | ddPCR | cze |
| dc.subject | DNA Fragmentation | cze |
| dc.subject | 16S rRNA | cze |
| dc.subject | end-point detection | cze |
| dc.subject | Python | cze |
| dc.subject | correction factor | cze |
| dc.subject | ddPCR | eng |
| dc.subject | DNA Fragmentation | eng |
| dc.subject | 16S rRNA | eng |
| dc.subject | end-point detection | eng |
| dc.subject | Python | eng |
| dc.subject | correction factor | eng |
| dc.title | <i><sklopený text></i>In silico modelling of the effect of bacterial DNA fragmentation on gene counts using digital droplet PCR | cze |
| dc.title.alternative | <i><sklopený text></i>In silico modelling of the effect of bacterial DNA fragmentation on gene counts using digital droplet PCR | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 69857 | |
| dc.description.abstract-translated | The aim of this study is to establish a correction factor for ddPCR, accounting for instances of the target sequence's occurrences being miscounted due to endpoint detection limitations. | eng |
| dc.date.accepted | 2022-09-15 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biological Chemistry | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biological Chemistry | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.description.defence | <p>The head of the commission, Prof. vácha, welcomed the student, commission members, and the audience. The supervisor Dr. Angel was not present. The student presented information on the ddPCR technique, 16S RNA use and in silico PCR. The setup for analyses was presented, followed by the results.</p>
<p> The supervisor's review was read by Dr. Petrová. Then the opponent presented her review, and the student answered the questions. Follow-up questions on the format of the thesis and script presentation were mostly answered, as well question on the AT, and GC content. Comments on the thesis and the discussion were presented, especially the format of the thesis was critised.</p>
<p>3x excellent, 1x very good, 1x good, final very good.</p> | cze |
