| dc.contributor.advisor | Franta, Zdeněk | |
| dc.contributor.author | Schmelzer, Jakob | |
| dc.date.accessioned | 2026-01-06T11:54:11Z | |
| dc.date.available | 2026-01-06T11:54:11Z | |
| dc.date.issued | 2023 | |
| dc.date.submitted | 2022-12-15 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/48491 | |
| dc.description.abstract | Tick-borne encephalitis virus (TBEV) is an important pathogen of the family Flaviviridae with an emerging health concern. Virus causes severe neurological disorders to human population in Europa and Asia. Despite effective vaccine, there is no specific antiviral treatment till now. The capsid protein encapsules the viral positive-sense single-stranded RNA and is essential in packaging and assembling of new virions within the host. A deeper understanding of these interactions and their implications should lead to new antiviral strategies and drug design. This thesis deals with the expression and purification of the capsid protein without membrane anchor using Escherichia coli expression system. | cze |
| dc.format | 44 | |
| dc.format | 44 | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | Flavivirus | cze |
| dc.subject | Tick-borne encephalitis virus | cze |
| dc.subject | Capsid protein | cze |
| dc.subject | Cloning | cze |
| dc.subject | Expression | cze |
| dc.subject | IPTG | cze |
| dc.subject | BL21-CodonPlus (DE3)-RIPL | cze |
| dc.subject | Immobilized Metal Affinity Chromatography | cze |
| dc.subject | Flavivirus | eng |
| dc.subject | Tick-borne encephalitis virus | eng |
| dc.subject | Capsid protein | eng |
| dc.subject | Cloning | eng |
| dc.subject | Expression | eng |
| dc.subject | IPTG | eng |
| dc.subject | BL21-CodonPlus (DE3)-RIPL | eng |
| dc.subject | Immobilized Metal Affinity Chromatography | eng |
| dc.title | Expression and purification of Tick-borne encephalitis virus Capsid protein | cze |
| dc.title.alternative | Expression and purification of Tick-borne encephalitis virus Capsid protein | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 60749 | |
| dc.description.abstract-translated | Tick-borne encephalitis virus (TBEV) is an important pathogen of the family Flaviviridae with an emerging health concern. Virus causes severe neurological disorders to human population in Europa and Asia. Despite effective vaccine, there is no specific antiviral treatment till now. The capsid protein encapsules the viral positive-sense single-stranded RNA and is essential in packaging and assembling of new virions within the host. A deeper understanding of these interactions and their implications should lead to new antiviral strategies and drug design. This thesis deals with the expression and purification of the capsid protein without membrane anchor using Escherichia coli expression system. | eng |
| dc.date.accepted | 2023-01-23 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biological Chemistry | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biochemistry | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.description.defence | <p>Prof. Grubhoffer welcome the student members of the comission. All members of the commission were present. Mr. Schmelzer described the studied virus - tick-borne encephalitis virus, and its capsid protein. The student described the various expression protocols and the results obtained. The supervisor and the opponent presented their reviews. Student answered the questions. Then, he answered questions from the audience, on expression of proteins, the use of anchor in recombinant proteins.</p>
<p>1x excellent, 3x very good, 1x good = very good</p> | cze |
