| dc.contributor.advisor | Daebeler, Anne | |
| dc.contributor.author | Zuser, Christina | |
| dc.date.accessioned | 2026-01-06T11:54:28Z | |
| dc.date.available | 2026-01-06T11:54:28Z | |
| dc.date.issued | 2023 | |
| dc.date.submitted | 2023-08-17 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/20.500.14390/48548 | |
| dc.description.abstract | The significance of methane as an energy source is discussed, specifically focusing on understanding the ecology and metabolism of methanotrophs belonging to the families Methylococcaceae, Methylocystaceae, and Beijerinckiaceae. It includes applying bioinformatic tools, primer design, and a comparative analysis of metagenome-assembled genomes using the NCBI database, shedding light on phyla representation discrepancies. The study contributes to understanding methanotrophic diversity. | cze |
| dc.format | 35 p. (68 075 characters) | |
| dc.format | 35 p. (68 075 characters) | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | Methanotrophs | cze |
| dc.subject | sMMO (soluble methane monooxygenase) | cze |
| dc.subject | Methylococcaceae | cze |
| dc.subject | Methylocystaceae | cze |
| dc.subject | Beijerinckiaceae | cze |
| dc.subject | Primer design | cze |
| dc.subject | Metagenome-Assembled Genomes (MAGs) | cze |
| dc.subject | Phylogenetic analysis | cze |
| dc.subject | Methanotrophs | eng |
| dc.subject | sMMO (soluble methane monooxygenase) | eng |
| dc.subject | Methylococcaceae | eng |
| dc.subject | Methylocystaceae | eng |
| dc.subject | Beijerinckiaceae | eng |
| dc.subject | Primer design | eng |
| dc.subject | Metagenome-Assembled Genomes (MAGs) | eng |
| dc.subject | Phylogenetic analysis | eng |
| dc.title | Curated Database Construction for sMMO-Containing Methanotrophs | cze |
| dc.title.alternative | Curated Database Construction for sMMO-Containing Methanotrophs | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 67310 | |
| dc.description.abstract-translated | The significance of methane as an energy source is discussed, specifically focusing on understanding the ecology and metabolism of methanotrophs belonging to the families Methylococcaceae, Methylocystaceae, and Beijerinckiaceae. It includes applying bioinformatic tools, primer design, and a comparative analysis of metagenome-assembled genomes using the NCBI database, shedding light on phyla representation discrepancies. The study contributes to understanding methanotrophic diversity. | eng |
| dc.date.accepted | 2023-09-20 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biological Chemistry | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biological Chemistry | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Kyslík, Jiří | |
| dc.description.defence | <p>Dr Štěrba welcomed the student and commission members; all members of the commission were present. The student presented the theoretical background of her work on methanotrophs and methane monooxygenases. Then, the experimental approach and the results were presented. The supervisor, Dr Daebeler, and the opponent, Dr Kyslík, presented their reviews. The student answered all the questions of the opponent and also questions from the commission members on how these genes are conservative in the genomes, real-life methodological setup compared to the performed in silico analysis, and the possibility of detecting all mmox genes in one or more reactions, environmental conditions of the organisms from which the sequences were analyzed, if whole gene or partial seuqnces were used for searching and the correctness of sequences in databases.<br />
Votes: 3x 1, 2x 2<br />
Final grade: 1<br />
Points: 130</p> | cze |
