Preparation of Specimen for Transmission Electron Microscopy using Freeze Substitution and Agitation

Grahammer, Johannes

dc.contributor.advisor	Vancová, Marie
dc.contributor.author	Grahammer, Johannes
dc.date.accessioned	2022-03-08T14:21:55Z
dc.date.available	2022-03-08T14:21:55Z
dc.date.issued	2018
dc.date.submitted	2018-05-14
dc.identifier.uri	https://dspace.jcu.cz/handle/20.500.14390/38506
dc.description.abstract	The quality of biological samples for Transmission Electron Microscopy prepared using High Pressure Freezing and Freeze Substitution with a novel agitation module invented by Helmuth Goldhammer and Siegfried Reipert was compared to Cells prepared without agitation and the Freeze Substitution protocol by Obornik et al. The amount of extraction was quantified via immunolabelling of RuBisCO inside the plastids of the Alveolate Chromera Velia Mechanical Damages were quantified by counting of obviously damaged and intact cells at low magnification	cze
dc.format	34
dc.format	34
dc.language.iso	eng
dc.publisher	Jihočeská univerzita	cze
dc.rights	Bez omezení
dc.subject	Freeze Substitution	cze
dc.subject	High Pressure Freezing	cze
dc.subject	Agitation	cze
dc.subject	Immunolabeling	cze
dc.subject	RuBisCO	cze
dc.subject	Freeze Substitution	eng
dc.subject	High Pressure Freezing	eng
dc.subject	Agitation	eng
dc.subject	Immunolabeling	eng
dc.subject	RuBisCO	eng
dc.title	Preparation of Specimen for Transmission Electron Microscopy using Freeze Substitution and Agitation	cze
dc.title.alternative	Preparation of Specimen for Transmission Electron Microscopy using Freeze Substitution and Agitation	eng
dc.title.alternative	-	cze
dc.type	bakalářská práce	cze
dc.identifier.stag	49787
dc.description.abstract-translated	The quality of biological samples for Transmission Electron Microscopy prepared using High Pressure Freezing and Freeze Substitution with a novel agitation module invented by Helmuth Goldhammer and Siegfried Reipert was compared to Cells prepared without agitation and the Freeze Substitution protocol by Obornik et al. The amount of extraction was quantified via immunolabelling of RuBisCO inside the plastids of the Alveolate Chromera Velia Mechanical Damages were quantified by counting of obviously damaged and intact cells at low magnification	eng
dc.date.accepted	2018-06-18
dc.description.department	Přírodovědecká fakulta	cze
dc.thesis.degree-discipline	Biological Chemistry	cze
dc.thesis.degree-grantor	Jihočeská univerzita. Přírodovědecká fakulta	cze
dc.thesis.degree-name	Bc.
dc.thesis.degree-program	Biochemistry	cze
dc.description.grade	Dokončená práce s úspěšnou obhajobou	cze
dc.contributor.referee	Nebesářová, Jana