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Hafnium Chloride, an Alternative Staining Reagent for Biological Electron Microscopy

dc.contributor.advisorVancová, Marie
dc.contributor.authorBaranyi, Magdalena Victoria
dc.date.accessioned2024-03-12T11:34:42Z
dc.date.available2024-03-12T11:34:42Z
dc.date.issued2021
dc.date.submitted2020-12-09
dc.identifier.urihttps://dspace.jcu.cz/handle/20.500.14390/44733
dc.description.abstractThis study focuses on the staining pattern of the non-radioactive heavy metal EM stain HfCl4, used during freeze substitution specimen preparation. HfCl4 was investigated as an alternative for the prominent uranyl acetate, since uranium based materials have been placed under heavy restrictions and bans worldwide. We have found a strong HfCl4 staining pattern of microtubules in myoepithelial cells of Ixodes ricinus salivary glands. Additionally, in several samples, HfCl4 was found to completely fill the cytoplasm of the myoepithelial cells. Nonetheless, artefact formation around granulated cells was also experienced.cze
dc.language.isoeng
dc.publisherJihočeská univerzitacze
dc.rightsBez omezení
dc.subjectelectron microscopycze
dc.subjecttransmission electron microscopycze
dc.subjectfixationcze
dc.subjectstainingcze
dc.subjecturanyl acetatecze
dc.subjecthafnium chloridecze
dc.subjectradioactivecze
dc.subjectixodes ricinuscze
dc.subjectsalivary glandscze
dc.subjectimagingcze
dc.subjectmicrotubulescze
dc.subjectmyoepithelial cellcze
dc.subjectcytoplasmcze
dc.subjectelectron microscopyeng
dc.subjecttransmission electron microscopyeng
dc.subjectfixationeng
dc.subjectstainingeng
dc.subjecturanyl acetateeng
dc.subjecthafnium chlorideeng
dc.subjectradioactiveeng
dc.subjectixodes ricinuseng
dc.subjectsalivary glandseng
dc.subjectimagingeng
dc.subjectmicrotubuleseng
dc.subjectmyoepithelial celleng
dc.subjectcytoplasmeng
dc.titleHafnium Chloride, an Alternative Staining Reagent for Biological Electron Microscopycze
dc.title.alternativeHafnium Chloride, an Alternative Staining Reagent for Biological Electron Microscopyeng
dc.typebakalářská prácecze
dc.identifier.stag53034
dc.description.abstract-translatedThis study focuses on the staining pattern of the non-radioactive heavy metal EM stain HfCl4, used during freeze substitution specimen preparation. HfCl4 was investigated as an alternative for the prominent uranyl acetate, since uranium based materials have been placed under heavy restrictions and bans worldwide. We have found a strong HfCl4 staining pattern of microtubules in myoepithelial cells of Ixodes ricinus salivary glands. Additionally, in several samples, HfCl4 was found to completely fill the cytoplasm of the myoepithelial cells. Nonetheless, artefact formation around granulated cells was also experienced.eng
dc.date.accepted2021-01-26
dc.description.departmentPřírodovědecká fakultacze
dc.thesis.degree-disciplineBiological Chemistrycze
dc.thesis.degree-grantorJihočeská univerzita. Přírodovědecká fakultacze
dc.thesis.degree-nameBc.
dc.thesis.degree-programBiochemistrycze
dc.description.gradeDokončená práce s úspěšnou obhajoboucze
dc.contributor.refereeTýč, Jiří


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